Analysis of thyroid-selective Glis3-Pax8Cre(LID) mice, which do not develop hyperinsulinemia, showed that in contrast to ubiquitous Glis3-KO(LID) mice, thyroid follicular cell proliferation, expression of cell cycle genes, including Ccnb1, Ccnb2, and Cdca2, and activation of the mTOR, a pathway that promotes cell proliferation, were not repressed in Glis3-Pax8Cre(LID) mice but induced to a similar degree as in WT(LID) mice (Figs. 5C– F and 6C). Here, GLIS3 is linked to hyperinsulinism.