In addition, we found that Wnt3a, a ligand of the Wnt/β-catenin signaling pathway, was upregulated in the lesion tissues of IBD patients and colitis mice, and TRPM8 can inhibit the combination of PKAca molecule of cAMP signaling pathway and GSK-3β molecule of Wnt/β-catenin signaling pathway, thereby inhibiting GSK-3β phosphorylation. Here, GSK3B is linked to colitis.