For i.v. injection of aPDL1 and transdermal delivery of CS/aPDL1 groups, the tumor infiltrations of both CD4+ and CD8+ T cells, as well as the expressions of granzyme B, Ki67, and IFN-γ, remained nearly unchanged, which might be resulted from the low tumor accumulation of aPDL1 after intravenous injection or transdermal delivery using CS. This evidence concerns the gene CD4 and neoplasm.