Using clinical human PDAC samples, we validated the protein expression patterns of selected genes in tumor and adjacent normal area, including AHNAK2, AREG, SEMA3A, SNCG, and WNT10A. Particularly, AHNAK2 was previously proposed as a PDAC prognosis biomarker42, corroborating the robustness of our findings. Here, AREG is linked to neoplasm.