Defactinib (10 μM) effectively inhibited the invasion of KYSE410 or KYSE510 cells in ESCC cells/CAFs #1 harbored AKT2 S128A or CCTα S315/319/323A mutant coculture system, compared with that of defactinib in KYSE410 or KYSE510/CAFs #1 harbored control vector coculture system (Fig. 5b). This evidence concerns the gene AKT2 and esophageal squamous cell carcinoma.