Vascular Smooth Muscle Cells (VSMCs) and monocytes/macrophages have been also proved to be involved in the process of atherosclerosis, but our immunofluorescence staining results did not show that AAV-shRNA Ash2l had an effect on the expression of Ash2l in VSMCs and monocytes/macrophages, which indicated that AAV-shRNA Ash2l was targeted at ECs (Fig. S1A and B). The gene discussed is ASH2L; the disease is atherosclerosis.