To study the effect of Ash2l ablation on the development of atherosclerosis, Ash2l in ECs was knocked down using ECs-specific Ash2l shRNA adeno-associated virus (AAV-shAsh2l) to infect ApoE−/− mice, and AAV-shRNA Control (AAV-Ctrl) was injected as control. This evidence concerns the gene APOE and atherosclerosis.