We also evaluated the relationship between cell number in different subtypes of T cells and tumor TCR repertoire, and found that TCR clonality correlated only with the cell numbers of CD3 + (r = 0.6748; p = 0.0006; Fig. 6B) and CD8 + cells (r = 0.5414; p = 0.0092; Fig. 6D), but not with CD4 + cells (r = 0.2017; p = 0.3681; Fig. 6C), suggesting that T cell clonality in PSCC may be mainly driven by the clonal expansion of CD8 + T cells. Here, CD4 is linked to neoplasm.