CCK‐8 assay, EdU assay and colony‐formation assay showed that overexpression of AKT could reverse the inhibition of HCC proliferation caused by decreasing USP27X‐AS1 (Figure 6A,B,E,F,I,J and Figure S8I,K), while USP27X‐AS1 lost the ability to promote HCC proliferation upon decreasing AKT (Figure 6C,D,G,H,K,L and Figure S8J,L). The gene discussed is AKT1; the disease is hepatocellular carcinoma.