Our data indicated that treatment of both melanoma and CRC cells with AHE and AHE-2 extracts resulted in significant suppression of several cell cycle regulator expressions, including CDK4, CDK6, p-Rb, E2F1, and Cyclin A, which in turn inhibited cell proliferation and arrested the cell cycle at the S phase. This evidence concerns the gene E2F1 and colorectal carcinoma.