Our analysis indicated that administrating CHIR99021 at 30 min did not influence the recruitment of leukocytes, neutrophils, Ly-6C+ monocytes, interstitial macrophages, T cells, CD4+ T cells, and CD8+ T cells (Figure 3a) at 12 h after LPS-induced ALI. The gene discussed is CD4; the disease is acute respiratory distress syndrome.