Since the Wnt/β-catenin signaling pathway was previously reported as a vital regulatory factor in cholangiocarcinoma [24–26], the key pathway-related proteins, including Wnt3a, phosphorylated GSK3β, and β-catenin, were detected in HCCC-9810 cells after FOXP1 overexpression or knockdown. This evidence concerns the gene FOXP1 and cholangiocarcinoma.