To confirm the novel finding that cryptic peptides reflecting TDP-43 loss of function are detectable in presymptomatic disease, we analyzed an additional cohort of largely presymptomatic C9orf72 mutation carriers from the Dominant Inherited ALS (DIALS) Network to further characterize early loss of TDP-43 splicing repression. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.