To investigate the function of AP2XI-2 in bradyzoites, the frequency of tachyzoite-to-bradyzoite differentiation in the AP2XI-2-mAID parasites incubated under alkaline growth conditions with or without IAA, was examined by using FITC-Dolichos biflorus lectin (DBL) staining, which binds to N-acetylgalactosamine in the parasite cyst wall22. The gene discussed is MCF2; the disease is cyst.