Super‐resolution microscopy (Figure 2A1) and Imaris quantification (Figure 2A2‐A4) of VGlut1, PSD95, and VGlut1‐PSD95 colocalized synaptic puncta showed a significant VGlut1 presynaptic loss (55% reduction) in the Arctic mouse model of AD (vs WT). This evidence concerns the gene DLG4 and Alzheimer disease.