Interestingly, we found unexpectedly high expression of nine m5C regulators in the tumor group (NOP2, NSUN3, NSUN6, NSUN7, DNMT1, DNMT3A, TRDMT1, TET2, and TET3), indicating their essential role in AML pathogenesis (Figure 2A). This evidence concerns the gene DNMT3A and acute myeloid leukemia.