IFN-gamma and IL-17a are mainly expressed through natural infection or wP vaccination, but poorly expressed by aP vaccination [27,28,63], and long term protection from the wP vaccination could be explained with it.Assuming that stimulation with heat hBp mimics the response to natural infection [44], the highest responses of IFN-gamma and IL-17a to hBp stimulation are consistent with the results of a previous study conducted in our laboratory [44]. Here, IL17A is linked to infection.