We speculated that celastrol might regulate lipogenic and lipid transport to ameliorate hepatic steatosis in cluster of differentiation 36 (CD36), medium chain acyl-CoA dehydrogenase (MCAD), acyl-CoA oxidase (ACOX), stearyl-CoA desaturase-1 (SCD1), fatty acid desaturase 2 (Fads2), acetyl-CoA carboxylase1 (Acc1), and fatty acid synthase (Fasn). This evidence concerns the gene CD36 and fatty liver disease.