However, we can see via immunofluorescence that C6 glioma cells express HCAR1 (Figure 6A), and we compared this cell type with a primary astrocyte culture via Western blotting, finding that C6 glioma cells have more HCAR1 content than primary astrocyte cultures (Figure 6B, p = 0.0168). This evidence concerns the gene HCAR1 and glioma.