We evaluated protein production of a subset of these factors in BCs cultures from additional WT and CF donors as described above and confirmed that Pf4 stimulation strongly increases production of CXCL1, CXCL8, and CXCL5 above the level induced by LPS alone in both donors (Figure 6D), though the magnitude of response was stronger in WT cells. This evidence concerns the gene CXCL5 and cystic fibrosis.