To determine whether the inhibition of HCC cells by Ezetimibe is mediated through apoptosis, Annexin V-APC/7-AAD staining and flow cytometry were performed on SNU-449 and HepG2 cells treated with various doses (0 to 75 μM) of Ezetimibe for 48 h. This evidence concerns the gene ANXA5 and hepatocellular carcinoma.