To validate whether the candidate gene PPP4R3A Asp409Asn mutation conforms to the basic inheritance pattern of FNMTC, we performed validations within the family, between families, in sporadic thyroid cancer cases, and the healthy population, following the gene mapping strategy outlined in Figure 2A. For this particular family, we conducted intrafamilial validation on members I 4, II 4, and III 9. Here, PPP4R3A is linked to thyroid gland carcinoma.