The multiplex assay of aberrantly methylated seven genes (RASSF1A, RUNX3, TFPI2, OPCML, secreted frizzled-related protein 5-SFRP5, cadherin 1-CDH1, and APC regulator of WNT signaling pathway-APC) achieved a sensitivity of 85% and a specificity of 90% in early-stage ovarian cancer, which was surprisingly better than the result obtained for CA125 [200]. This evidence concerns the gene SFRP5 and ovarian cancer.