Epithelial cell adhesion molecule EpCAM+/cytokeratin+, CD45-, and 4’,6-diamidino-2-phenylindole (DAPI)+ CTCs counts used for the serial monitoring of 13 ovarian cancer patients (10 with HGSOC and 11 with complete response to first-line chemotherapy) showed higher sensitivity (100.0% vs. 60.0%), positive predictive values (55.6% vs. 42.9%), and negative predictive values (100.0% vs. 87.5%) than CA125 levels. Here, EPCAM is linked to ovarian cancer.