Objective: The primary objective of this study was to check whether antioxidant vitamins (C and E) can reduce hyperglycemia and hyperinsulinemia in response to glyphosate exposure and the secondary objective was to investigate whether antioxidant vitamins have the capacity to downregulate GSK-3β and FOXO-1-mediated oxidative stress in the liver of glyphosate induced rats. The gene discussed is GSK3B; the disease is hyperinsulinism.