Objective: The primary objective of this study was to check whether antioxidant vitamins (C and E) can reduce hyperglycemia and hyperinsulinemia in response to glyphosate exposure and the secondary objective was to investigate whether antioxidant vitamins have the capacity to downregulate GSK-3β and FOXO-1-mediated oxidative stress in the liver of glyphosate induced rats. This evidence concerns the gene FOXO1 and hyperinsulinism.