The incidence of AFP >20 μg/L in the DPHCC group was significantly higher than that in the ICC group (P = 0.000), whereas the incidence rates of CA19-9 >37 kU/L and CA125 >35 kU/L in the DPHCC group were significantly lower than that in the ICC group. This evidence concerns the gene AFP and intrahepatic cholangiocarcinoma.