Performing traditional dewaxing, rehydration, antigen retrieval, overnight antibody incubation, and subsequent photocleavage of the light sensitive probes, we profiled the tubulovillous adenoma, stage I, and stage IV CRC tissues with immune specific antibodies of CD20 (B-cells), CD11b (monocytes), CD3 (T-cells), and CD44 (T-cells), and the extracellular matrix component of Collagen 1A1 (blue in whole tissue image) for structural information. This evidence concerns the gene CD44 and tubulovillous adenoma.