To characterize the dynamics of DDX3X-dependent changes in the gene expression cascade, we employed a previously validated auxin-degron system to efficiently deplete DDX3X protein in the HCT116 colorectal cancer cell line (Venkataramanan et al, 2021), where we found near-complete rescue of gene expression changes by DDX3X expression, thus being able to use this tool to monitor DDX3X-dependent changes. Here, DDX3X is linked to colorectal cancer.