PDGFA and central nervous system cancer: Additionally, we developed a genetically engineered murine glioma model, leveraging RCAS/Ntv-a retroviral transduction to express the oncogene platelet-derived growth factor A (PDGFA), an shRNA targeting Tp53, and Cre-recombinase in nestin-positive cells within the brains of isogenic mice harboring either intact or floxed Atrx loci28.