Furthermore, in vitro functional assays using HBMEC endothelial cells, coupled to 2D and 3D GBM cells culture system, confirmed that the secretome obtained from iHDAC treated GBM cells drastically disrupted endothelial cells behavior, impairing migratory capacity of endothelial cells coupled to a deficient tubulogenesis in a Fibronectin and VEGF independent fashion. The gene discussed is VEGFA; the disease is glioblastoma.