The transcript landscape in allografts subjected to prolonged CIS versus minimal CIS with or without p40HD stimulation reflects the gene expression differences in allografts with minimal donor-reactive memory CD8+ T cell activation, allografts with memory CD8+ T cell proliferation without expression of effector functions to mediate acute injury, and allografts with both memory CD8+ T cell proliferation and strong expression of effector function to mediate acute graft injury. This evidence concerns the gene CD8A and in situ carcinoma.