To test whether allograft DCs were required for p40HD-induced endogenous memory CD8+ T cell proliferation within allografts subjected to minimal CIS, we treated B6.CD11c-DTR and wild-type B6 heart allograft donors with diphtheria toxin (DT) on the day prior to graft harvest and minimal CIS before transplant to complete-MHC-mismatched A/J recipients and administered p40HD as above. The gene discussed is ITGAX; the disease is in situ carcinoma.