Meanwhile, MSCs with Mut p53 are considered the cells of origin of bone tumors, affecting osteogenic differentiation and influencing the properties of osteosarcoma TME components such as inducible nitric oxide synthase, CCL5, IL-6 and TGF-β expression at higher levels, ultimately promoting osteosarcoma development [75]. This evidence concerns the gene TGFB1 and osteosarcoma.