To elucidate the downstream targets modulated by FTO in ccRCC, stable low-expressing FTO cell lines (769-P LV-SH FTO) were established by transfecting plasmids carrying FTO shRNA into 769-P cells, followed by a two-week selection period with 1 μg/ml puromycin. Here, FTO is linked to nonpapillary renal cell carcinoma.