Therefore, high level of AMA‐M2 can be detected in serum of PBC patients, this result is also consistent with the study of Han et al.24Serum level of anti‐gp210 and anti‐sp100 antibodies in PBC group were significantly higher than those in other disease groups and healthy controls, while there was no significant difference between other disease and healthy control groups. The gene discussed is NUP210; the disease is primary biliary cholangitis.