Among the 13 m5C regulators, nine (DNMT3A‐3B, DNMT1‐2, NSUN1‐7, DNMT3A, and NSUN5) were significantly related to prognosis of 417 AML samples derived from GEO database (GSE37642 data set). This evidence concerns the gene NSUN5 and acute myeloid leukemia.