When VWF:RCo/VWF:Ag of <0.7 is used for the diagnosis of AS-induced AVWS, we need to consider following: patients with PAD exhibited the VWF:RCo/VWF:Ag of around 0.8, which is close to 0.7; VWF:RCo/VWF:Ag ratios were correlated with the severity of AS although the tilt angle of the slope of the regression line was rather low, compared to that of VWF large multimer indices. The gene discussed is VWF; the disease is aortic stenosis.