METTL1 and acute myeloid leukemia: To explore whether the knockdown of METTL1 could increase the overall levels of tsRNA in AML cells, we performed the high-throughput small noncoding RNA sequencing experiment between METTL1 knockdown and METTL1 control AML cells, the results showed that the levels of some tsRNAs, including 5’tsRNA, inner’ tsRNAs, and 3’CCA-tsRNAs are remarkably increased in METTL1 knockdown AML cells (Fig. 7B), which were further confirmed by northern blot and RT-PCR (Fig. 7C–E).