To further confirm whether the translation inhibition in METTL1 knockdown AML cells was induced by m7G dysregulated tRNA methylome, we first performed northern blot to detect the levels of some selected tRNAs in AML cells, the data showed that the levels of tRNAAla, tRNAVal, and tRNALeu were significantly decreased in METTL1 knockdown AML cells compared to METTL1 control AML cells (Fig. 6A–D), suggesting that the absence of METTL1 mediated tRNA m7G might affect the cellular tRNA spectrum in AML cells. This evidence concerns the gene METTL1 and acute myeloid leukemia.