In addition, we detected that the proportion of CD11b-stained leukemic cells from PB, BM, and spleen samples was significantly increased in Fto-knockdown and FB23-2-treated recipient mice compared with that in the vehicle control (Fig. 3P–R and Additional file 1: Fig. S3K–M), suggesting that inhibition of FTO promoted the differentiation of t(8;21) AML cells in vivo. This evidence concerns the gene FTO and acute myeloid leukemia.