Analysis of peripheral T-cells using flow cytometry revealed an increase in PD-1 expression on CD4 + and CD8 + T-cells in TOFU-DCs + PD-1 treated Kluc (Fig. 4a) and GL261 (Fig. 4b) tumor-bearing mice supporting systemic activation of T-cells as previously described [56–58]. The gene discussed is CD4; the disease is neoplasm.