To understand the intra-tumoral immune cell response induced by ACT employing TOFU mRNA encoded antigens, and identify potential interventions that could overcome treatment resistance, we performed single cell (sc) RNA-seq on CD45 + immune cells and CD45 − tumor and normal brain cells that were isolated from the tumors of mice treated with TOFU-ACT or control treatments (Fig. 6a) [44]. This evidence concerns the gene PTPRC and neoplasm.