To examine the possible involvement of mitochondria in pyroptosis of CD4+T cells in HIV infection, we first measured the MM of freshly isolated PBMC from UNT, ART and HD using a mitochondrial specific dye (MitoLiteTM Orange FM), which binds the mitochondrial membrane independently of the membrane potential and gives an index of mitochondrial mass based on staining intensity [12, 24]. Here, CD4 is linked to HIV infectious disease.