To determine whether PD-L2 is also upregulated in primary cultures from patients with cancer when cells undergo TIS, we established cultures from head and neck cancer (VHIO-008), endometrial cancer (VHIO-35035) and melanoma (VHIO-088) treated with the chemotherapeutic agent bleomycin. The gene discussed is PDCD1LG2; the disease is head and neck cancer.