In the future we would like to generate additional SOX2 knock-in lines covering a range of different GBM subtypes, including mesenchymal GSCs which associate better with a quiescent phenotype57,58, but also a healthy human neural stem cell line counterpart to examine if and how the SOX2 expression pattern is altered between a malignant and non-malignant cell line. Here, SOX2 is linked to glioblastoma.