Herein, MSU-induced gouty arthritis, an acute NLRP3-related inflammatory model was used to clarify the function of HECTD3 in vivo. In the MSU-induced gout model, mice were intra-articular injected with 1 mg of MSU to activate NLRP3 inflammasome-dependent arthritis. The gene discussed is NLRP3; the disease is gout.