Comparing these parameters in the presence of basal glucagon to GCG-DF with the combination of hyperglycemia under basal insulin (ZCL rats; Fig. 1, protocol 6 and ZDF rats; Fig. 1, protocol 8), all measured parameters in GCG-DF ZDF rats were not significantly different from those under GCG-ZDF-BC (Fig. 5). This evidence concerns the gene INS and Hyperglycemia.