To validate the scRNA‐seq data, a cohort of independent samples was subjected to mIHC, and quantitative analysis of the imaging data showed good agreement with scRNA‐seq, such as increased myeloid cells (CD11C+ cells) in SJP polyps and increased epithelial cells (EpCAM+ cells) in PJS polyps compared with normal tissues. The gene discussed is ITGAX; the disease is Peutz-Jeghers syndrome.