However, the increased expression levels induced by the HFD in the OFB implicates Spp1 as an important factor in the generation of a pro- inflammatory premetastatic niche; as a ligand of integrins and CD44 its presence in the extracellular matrix can also support cancer cell adhesion and subsequent metastatic outgrowth similar to COL1and FN1. This evidence concerns the gene FN1 and cancer.