Furthermore, Two lymphocyte subsets were identified to be significantly associated with GAD risk: CD24+ CD27+ B cell (OR=1.066,95%CI=1.04~1.10,P=1.237×10−5),CD28+CD4+T cell (OR=0.927, 95%CI=0.89~0.96, P=8.085×10−5). The gene discussed is CD27; the disease is generalized anxiety disorder.