Among them, there were 3 significant pathways, namely, primary bile acid biosynthesis, cholesterol metabolism, and choline metabolism in cancer, that were significantly enriched in the J-DDGS group; there were 11 significant pathways (including arachidonic acid metabolism, mTOR signaling pathway, and D-arginine and D-ornithine metabolism, etc.)that were significantly enriched in the I-DDGS group; and there were 20 significant pathways (including linoleic acid metabolism, GnRH signaling pathway, and leishmaniasis, etc.)that were significantly enriched in the C-DDGS group (Figures 5A–C). The gene discussed is GNRH1; the disease is cancer.