SIRT3 and hyperinsulinemic hypoglycemia, familial, 4: Moreover, overexpression of SIRT3 promotes osteoclast formation, resulting in a 2.5-fold increase in the number of osteoclasts on the bone surface (94) Further investigation is warranted to explore the resistance to bone loss after estrogen deficiency due to OVX observed in SIRT3−/− mice (95).