Intriguingly, combination of LC and PD-1 antibody (Fig. 4j) resulted in greater tumor growth inhibition and longer survival time (Fig. 4k) and more CD8+ T cell infiltration and the expression of IFN-γ and CD107a than single PD-1 antibody treatment in B16 melanoma-bearing mice (Fig. 4l). The gene discussed is CD8A; the disease is neoplasm.