Emerging evidence suggests that a hepatocellular lipid imbalance always initiates and promotes an inflammation in the liver which in turn aggravates NAFLD progression.25 To comprehensively explore the impact of Zbtb18 on NAFLD development, we also investigated the changes in NLRP3 inflammasome’s assembly and activation, which plays a key role in the liver inflammation in hepatic Zbtb18 gain or loss models. Here, NLRP3 is linked to metabolic dysfunction-associated steatotic liver disease.