Moreover, gene set enrichment analysis (GSEA) of the above DEGs identified in Zbtb18 overexpressing MPHs and the liver from the NAFLD patients showed that “FXR signaling pathway” was positively correlated with Zbtb18 expression, suggesting that the expression of these DEGs may be driven by FXR-dependent mechanisms (Fig. 5e). The gene discussed is ZBTB18; the disease is metabolic dysfunction-associated steatotic liver disease.