An accumulation of reads in these 1 kb bins leads to high coverage in the bin and so, high coverage bins (HCBs) could indicate the presence of DNA palindromes.29,36 Indeed, HCBs were seen in known palindromic junctions on chr1 at RPRD2, ECM1, and CTSK in Colo320DM cell line DNA (Fig. 1B).29 To simulate low tumor fraction (TF) as expected from plasma cfDNA, Colo320DM DNA was diluted with IMR90 normal fibroblast DNA in vitro prior to GAPF. This evidence concerns the gene ECM1 and neoplasm.